Review

pe conjugated anti ccr2 (R&D Systems)

R&D Systems is a verified supplier

R&D Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

R&D Systems pe conjugated anti ccr2
Pe Conjugated Anti Ccr2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pe+conjugated+anti+ccr2/Rat+CCR2+PE-conjugated+Antibody/pmc12486214-235-32-34
Average 93 stars, based on 7 article reviews

pe conjugated anti ccr2 - by Bioz Stars, 2026-10

93/100 stars

Images

Blocking Assay:

Article Title: Neutral or Detrimental Effects of TREM2 Agonist Antibodies in Preclinical Models of Alzheimer’s Disease and Multiple Sclerosis
Article Snippet: Ainhoa Etxeberria,* Yun-An A. Shen,* Stephen Vito,* Sean M. Silverman, Jose Imperio, Guita Lalehzadeh, Allison L. Soung, Changchun Du, Luke Xie, Man Kin Choy, Yi-chun Hsiao, Hai Ngu, Chang Hoon Cho, Soumitra Ghosh, Gloriia Novikova, Mitchell G. Rezzonico, Rebecca Leahey, Martin Weber, Alvin Gogineni, Justin Elstrott, Monica Xiong, Jacob J. Greene, Kimberly L. Stark, Pamela Chan, Gillie A. Roth, Max Adrian, Qingling Li, Meena Choi, Weng Ruh Wong, Wendy Sandoval, Oded Foreman, Alicia A. Nugent, Brad A. Friedman, Shraddha Sadekar, Isidro Hötzel, David V. Hansen, Ben Chih,12 Tracy J. Yuen, Robby M. Weimer, Amy Easton, William J. Meilandt, and Christopher J. Bohlen Departments of Neuroscience, Biochemical and Cellular Pharmacology, Translational Imaging, Antibody Engineering, Pathology, Human Pathobiology and OMNI Reverse Translation, Bioinformatics, Preclinical and Translational Pharmacokinetics and Pharmacodynamics, and Microchemistry Lipidomics and Proteomics, Genentech, Inc., South San Francisco, California 94080

Suspension:

Incubation:

Staining:

Article Title: HTRA1-dependent proteolysis induces age-related retinal degeneration and exacerbates choroidal neovascularization
Article Snippet: Cells were resuspended in flow cytometry buffer (PBS containing 0.5% bovine serum albumin and 2 mM EDTA, pH 8.0) and incubated with anti-CD16/CD32 (BD Biosciences) for 30 min to block any nonspecific binding to Fc receptors. .. Retinal cells were then stained with PE-Cy7-conjugated anti-CD11b (BD Biosciences, clone M1/70), APC-conjugated anti-CD90.2 (BD Biosciences, clone 53-2.1), Alexa Fluor 700-conjugated anti-CD45 (BioLegend, clone 30-F11), FITC-conjugated anti-ST2 (MD Bioproducts, clone DJ8) and PE-conjugated anti-CCR2 (R&D Systems). .. To detect intracellular markers, the following fluorophore-conjugated antibodies were generated using antibody conjugation kits (Abcam) according to the manufacturer's instructions: PE-Cy7-conjugated anti-rhodopsin (Rho; EMD Millipore, clone 1D4), PE-conjugated anti-cone arrestin (CAR; EMD Millipore) and PerCP-Cy5.5-conjugated anti-GFAP (Thermo Fisher Scientific, clone GA5).

Article Title: HTRA1-dependent proteolysis induces age-related retinal degeneration and exacerbates choroidal neovascularization.
Article Snippet: Cells were resuspended in FC buffer (PBS containing 0.5% bovine serum albumin and 2 mM EDTA, pH 8.0) and incubated with anti-CD16/CD32 (BD) for 30 minutes to block any nonspecific binding to Fc receptors. .. Retinal cells were then stained with PE- Cy7–conjugated anti-CD11b (clone M1/70; BD), APC-conjugated anti-CD90.2 (clone 53–2.1; BD), Alexa Fluor 700–conjugated anti-CD45 (clone 30-F11; BioLegend), FITC-conjugated anti-ST2 (clone DJ8; MD Bioproducts), and PE-conjugated anti-CCR2 (R&D Systems). .. To detect intracellular markers, the following fluorophore-conjugated Abs were generated using Ab conjugation kits (Abcam) according to the manufacturer’s instructions: PE-Cy7– conjugated anti- rhodopsin (Rho; clone 1D4; EMD Millipore), PE-conjugated anti-cone arrestin (CAR; EMD

Article Title: Anti-interleukin-33 antibodies and uses thereof
Article Snippet: Primary retinal cells were resuspended in flow cytometry buffer (PBS containing 0.5% bovine serum albumin and 2 mM EDTA, pH 8) and incubated with anti-CD16/CD32 (BD Biosciences) for 30 min to block non-specific staining. .. Mouse retinal cells were stained with PE-CY7®-conjugated anti-CD11b (clone M1/70, BD Biosciences), APC-conjugated anti-CD90.2 (clone 53-2.1, BD Biosciences), ALEXA FLUOR® 700-conjugated anti-CD45 (clone 30-F11, BioLegend), FITC-conjugated anti-ST2 (clone DJ8, MD Bioproducts), PE-conjugated anti-CCR2 (R&D systems), and FITC-conjugated anti-Ly6C and PE-conjugated anti-CD115 (clone AFS98, eBioscience). .. Rat retinal cells were stained with PE-CY7®-conjugated anti-CD11b/c (clone OX-42, BD Biosciences), APC-conjugated anti-CD90 (clone OX-7, BD Biosciences), and/or ALEXA FLUOR® 700-conjugated anti-CD45 (clone OX-1, BioLegend).

Image Search Results

( A ) Schema of female ApoE –/– mice on an HFD with 150 mM SerBut or NaBut on a butyrate basis, or water ad libitum for 6 weeks. ( B ) Oil Red O (ORO) staining of aortic root plaque. ( C ) CD68 + IHC staining of aortic root plaque. Original magnification, ×20. ( D ) Representative images of immunofluorescent staining of aortic root. ( E ) Quantification of ORO + aortic root valve area. ( F ) Histological Stary scoring of plaque severity, ( G ) quantified CD68 + area, and ( H ) necrotic core of CD68 + IHC staining. ( I ) Quantified DAPI + , ( J ) DAPI + per plaque area, ( K ) iNOS + , and ( L ) iNOS + cells per plaque area in immunofluorescence stain. ( M – O ) Flow cytometry of immune cells infiltrating the aorta: ( M ) CD45 + total leukocytes, ( N ) CD11b + monocytes, and ( O ) CCR2 + CD11b + monocytes. ( P ) Water consumed by cages for the first 19 days of experiment. Data represent mean water consumed between 2 cages (replicates) as weight change of bottle converted to water volume. Error bars represent SD. ( Q ) Plasma IL-6 and ( R ) IFN-γ. ( S ) Blood flow cytometry of Ly6G + CD11b + circulating neutrophils. n = 5/cage and n = 10 mice per group. Data points represent individual mice displayed with median ± SEM. Statistical analyses were performed using a 1-way ANOVA with Tukey’s, Welch’s (if SDs were significantly different by Bartlett and Brown-Forsyth tests), or Kruskal-Wallis (if data were not normally distributed determined by Shapiro-Wilk test) post hoc test. P values less than 0.10 are shown. Outliers were removed by ROUT testing at Q = 1% in I – L . Scoring in C was done blinded. Data in C and F – H represent 2 independent pooled experiments: n = 10 for water and SerBut groups and n = 10 for NaBut groups in a single experiment. “B6,NC” denotes age-matched C57BL/6 mice on normal diet as non-statistical comparison to visualize healthy examples. Scale bars: 200 μm.

Journal: JCI Insight

Article Title: A prometabolite strategy inhibits cardiometabolic disease in an ApoE –/– murine model of atherosclerosis

doi: 10.1172/jci.insight.191090

Figure Lengend Snippet: ( A ) Schema of female ApoE –/– mice on an HFD with 150 mM SerBut or NaBut on a butyrate basis, or water ad libitum for 6 weeks. ( B ) Oil Red O (ORO) staining of aortic root plaque. ( C ) CD68 + IHC staining of aortic root plaque. Original magnification, ×20. ( D ) Representative images of immunofluorescent staining of aortic root. ( E ) Quantification of ORO + aortic root valve area. ( F ) Histological Stary scoring of plaque severity, ( G ) quantified CD68 + area, and ( H ) necrotic core of CD68 + IHC staining. ( I ) Quantified DAPI + , ( J ) DAPI + per plaque area, ( K ) iNOS + , and ( L ) iNOS + cells per plaque area in immunofluorescence stain. ( M – O ) Flow cytometry of immune cells infiltrating the aorta: ( M ) CD45 + total leukocytes, ( N ) CD11b + monocytes, and ( O ) CCR2 + CD11b + monocytes. ( P ) Water consumed by cages for the first 19 days of experiment. Data represent mean water consumed between 2 cages (replicates) as weight change of bottle converted to water volume. Error bars represent SD. ( Q ) Plasma IL-6 and ( R ) IFN-γ. ( S ) Blood flow cytometry of Ly6G + CD11b + circulating neutrophils. n = 5/cage and n = 10 mice per group. Data points represent individual mice displayed with median ± SEM. Statistical analyses were performed using a 1-way ANOVA with Tukey’s, Welch’s (if SDs were significantly different by Bartlett and Brown-Forsyth tests), or Kruskal-Wallis (if data were not normally distributed determined by Shapiro-Wilk test) post hoc test. P values less than 0.10 are shown. Outliers were removed by ROUT testing at Q = 1% in I – L . Scoring in C was done blinded. Data in C and F – H represent 2 independent pooled experiments: n = 10 for water and SerBut groups and n = 10 for NaBut groups in a single experiment. “B6,NC” denotes age-matched C57BL/6 mice on normal diet as non-statistical comparison to visualize healthy examples. Scale bars: 200 μm.

Article Snippet: In this study, antibodies used included those against CD16/CD32 (BioLegend, clone 93, catalog 101302), CCR2 (PE; R&D Systems, catalog FAB5538P), CD11b (BV605; BioLegend, clone M1/70, catalog 101257), CD11c (PE-Cy7; BD Biosciences, clone HL3, catalog 558079, BD Biosciences), CD19 (APC; BioLegend, clone 1D3, catalog 152412), CD3e (BUV395; clone 145-2C11, catalog 563565), CD45 (BV786; BD Biosciences, clone 30-F11, catalog 564225), F4/80 (BUV496; BD Biosciences, clone T45-2342, catalog 750644), I-A/I-E (PerCP-Cy5.5; BioLegend, clone M5/114.15.2, catalog 107626), Ly-6C (APC-Cy7; BioLegend, clone HK1.4, catalog 128026), and Ly-6G (FITC; eBioscience, clone 1AB, catalog 11-9668-80).

Techniques: Staining, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Clinical Proteomics, Comparison

Temporal changes in the number of skin myeloid cells after the resolution of CHS. (A, B) Representative flow cytometry plots of isolated naïve and healed ear skin cells on day 35 post-challenge. (A) CD11b + dermal myeloid cells were analyzed according to previous studies ( – ). CD11b + CD24 lo/- dermal myeloid cells in CD45 + Lineage - (Lin - : CD3ε - CD19 - Ly-6G - ) cells contained CD64 - Ly-6C - cells (cDC2) and remaining CCR2 + and CCR2 - populations. Based on Ly-6C and MHC-II expression, CCR2 + cells were divided into dermal monocytes (P1) and Ly-6C + (P2) and Ly-6C - (P3) MoDCs/CCR2 + macrophages. CCR2 - cells consisted of MHC-II lo/- (P4) and MHC-II hi (P5) macrophages. (B) DCs and LC populations were analyzed according to previous studies ( , ). CD45 + Lin - CD64 - Ly-6C - MHC-II hi cells consisted of CD24 + CD11b lo/- (cDC1), CD24 + CD11b + (LCs), CD24 - CD11b + (cDC2), and CD24 - CD11b - (DN cDCs). (C, D) The numbers of each cell lineage in naïve and healed ear skin were analyzed. (C) Absolute cell numbers were compared between days 37 and 318. (D) Temporal changes in the relative numbers of each myeloid cell population (expressed as a percentage relative to those in naïve skin) were analyzed at the indicated time points. Representative results are shown for experimental results conducted multiple times around days 37 and 100, while data from day 214 onward are from a single experiment. Data represent the mean ± S.E. (n = 5-6, each). * P < 0.05 [two-tailed paired (naïve vs healed) and unpaired t- test (days 37 vs 318)].

Journal: Frontiers in Immunology

Article Title: CD4 + skin resident memory T cells preferentially colocalize with dermal Folr2 hi macrophages in contact hypersensitivity

doi: 10.3389/fimmu.2025.1590687

Figure Lengend Snippet: Temporal changes in the number of skin myeloid cells after the resolution of CHS. (A, B) Representative flow cytometry plots of isolated naïve and healed ear skin cells on day 35 post-challenge. (A) CD11b + dermal myeloid cells were analyzed according to previous studies ( – ). CD11b + CD24 lo/- dermal myeloid cells in CD45 + Lineage - (Lin - : CD3ε - CD19 - Ly-6G - ) cells contained CD64 - Ly-6C - cells (cDC2) and remaining CCR2 + and CCR2 - populations. Based on Ly-6C and MHC-II expression, CCR2 + cells were divided into dermal monocytes (P1) and Ly-6C + (P2) and Ly-6C - (P3) MoDCs/CCR2 + macrophages. CCR2 - cells consisted of MHC-II lo/- (P4) and MHC-II hi (P5) macrophages. (B) DCs and LC populations were analyzed according to previous studies ( , ). CD45 + Lin - CD64 - Ly-6C - MHC-II hi cells consisted of CD24 + CD11b lo/- (cDC1), CD24 + CD11b + (LCs), CD24 - CD11b + (cDC2), and CD24 - CD11b - (DN cDCs). (C, D) The numbers of each cell lineage in naïve and healed ear skin were analyzed. (C) Absolute cell numbers were compared between days 37 and 318. (D) Temporal changes in the relative numbers of each myeloid cell population (expressed as a percentage relative to those in naïve skin) were analyzed at the indicated time points. Representative results are shown for experimental results conducted multiple times around days 37 and 100, while data from day 214 onward are from a single experiment. Data represent the mean ± S.E. (n = 5-6, each). * P < 0.05 [two-tailed paired (naïve vs healed) and unpaired t- test (days 37 vs 318)].

Article Snippet: The following antibodies were used for myeloid cell staining: anti-Ly-6C-FITC (HK1.4, BioLegend), CCR2-PE (475301, R&D systems), CD3ε-biotin (145-2C11, BD Biosciences, San Jose, CA), CD19-biotin (6D5, BioLegend), Ly-6G-biotin (1A8, BioLegend), CD24-PE-Cy7 (M1/69, BioLegend), Tim-4-PE-Cy7 (RMT4-54, BioLegend), Rat IgG2a/κ isotype control-PE-Cy7 (RTK2758, BioLegend), Folr2-APC (10/FR2, BioLegend), Rat IgG2a/κ isotype control-APC (RTK2758, BioLegend), CD45.2-APC-Vio770 (REA737, Miltenyi Biotec), MHC-II-BV421 (M5/114.15.2, BioLegend), CD64-BV605 (X54-5/7.1, BD Biosciences), and CD11b-BV786 (M1/70, BioLegend).

Techniques: Flow Cytometry, Isolation, Expressing, Two Tailed Test

$Folr2 hi macrophages are the major macrophage population in skin. (A) The expression of common macrophage marker genes in dermal myeloid cell lineages in mouse naïve ear skin was obtained from a public database . The heat map shows microarray expression values (color scale based on percentiles). (B-F) The frequency of Folr2 hi cells in naïve and healed ear skin cells on day 38 was analyzed. Representative plots of CD45 and Folr2 expression in whole ear skin cells (B) , and Folr2 expression in P1-P5, Lin + (CD3ε + CD19 + Ly-6G + ), and other remaining CD45 + cell fractions (C) . (D) Pie charts show the distribution of total Folr2 hi cells to the indicated fractions. The size of pie charts represents the number of cells. “Other CD45 + ” and “stroma” fractions represent CD45 + cells other than the P3-P5 fractions and CD45 - cells, respectively. (E) Distribution of Folr2 hi and Folr2 lo/- cells in total P3-P5 fractions. (F) Numbers of Folr2 hi and Folr2 lo/- cells in P3-P5 fractions. Data are the mean ± S.E. (n = 5, for each). * P < 0.05 (naïve left ears vs healed right ears, two-tailed paired t- test). (G) Classification of skin macrophage populations proposed by Dick et al. and found in the present study. (H) Tim-4, Folr2, and MHC-II expression in the CD45 + Lin - CD11b + CD64 + CCR2 - macrophage population in naïve and healed ear skin on day 50. Graphs represent the numbers of MHC-II hi and MHC-I lo/- populations in the indicated fractions (mean ± S.E., n = 5, for each). P values (naïve left ears vs healed right ears, two-tailed paired t- test). (F, H) The macrophage subset included in each population is shown at the bottom of the graphs.$

Journal: Frontiers in Immunology

Article Title: CD4 + skin resident memory T cells preferentially colocalize with dermal Folr2 hi macrophages in contact hypersensitivity

doi: 10.3389/fimmu.2025.1590687

Figure Lengend Snippet: Folr2 hi macrophages are the major macrophage population in skin. (A) The expression of common macrophage marker genes in dermal myeloid cell lineages in mouse naïve ear skin was obtained from a public database . The heat map shows microarray expression values (color scale based on percentiles). (B-F) The frequency of Folr2 hi cells in naïve and healed ear skin cells on day 38 was analyzed. Representative plots of CD45 and Folr2 expression in whole ear skin cells (B) , and Folr2 expression in P1-P5, Lin + (CD3ε + CD19 + Ly-6G + ), and other remaining CD45 + cell fractions (C) . (D) Pie charts show the distribution of total Folr2 hi cells to the indicated fractions. The size of pie charts represents the number of cells. “Other CD45 + ” and “stroma” fractions represent CD45 + cells other than the P3-P5 fractions and CD45 - cells, respectively. (E) Distribution of Folr2 hi and Folr2 lo/- cells in total P3-P5 fractions. (F) Numbers of Folr2 hi and Folr2 lo/- cells in P3-P5 fractions. Data are the mean ± S.E. (n = 5, for each). * P < 0.05 (naïve left ears vs healed right ears, two-tailed paired t- test). (G) Classification of skin macrophage populations proposed by Dick et al. and found in the present study. (H) Tim-4, Folr2, and MHC-II expression in the CD45 + Lin - CD11b + CD64 + CCR2 - macrophage population in naïve and healed ear skin on day 50. Graphs represent the numbers of MHC-II hi and MHC-I lo/- populations in the indicated fractions (mean ± S.E., n = 5, for each). P values (naïve left ears vs healed right ears, two-tailed paired t- test). (F, H) The macrophage subset included in each population is shown at the bottom of the graphs.

Techniques: Expressing, Marker, Microarray, Two Tailed Test

Review

pe conjugated anti ccr2 (R&D Systems)

Structured Review

Images

Related Articles

Blocking Assay:

Suspension:

Incubation:

Staining:

Similar Products

Image Search Results